Sequencing Services

SARS-CoV-2 WGS (ARTIC protocol) for rapid variant tracking.
Fast, reliable SARS-CoV-2 whole genome sequencing and lineage calling service for pathogen surveillance. We use the latest ARTIC primer set available (v5.3.2) to amplify the viral genome for the best results with currently circulating lineages. Library preparation is performed using a modified Illumina DNA Prep protocol and sequenced at 2x100 bp targeting a minimum of 5 million reads per sample.

Features:

• Processed fastq files following host removal and bam files aligned to the SARS-CoV-2 reference genome.
• Consensus SARS-CoV-2 genome fasta file.
• Lineage reports for samples with >70% genome coverage
• Two-week turnaround time or faster from initiation of sequencing run.

Sample Requirements:
• Purified RNA samples shipped to our Boston laboratory frozen on dry ice
• qPCR or dPCR values for SARS-CoV-2: Ct < 30 (qPCR) or > 300 copies/μL (dPCR) required for 70% coverage and lineage calling service.

Minimum sample volume: 40 μL of purified RNA

*** Ask us about our Influenza WGS and hybrid-capture panels, including Illumina's RPIP, RVOP and VSP v2 capabilities!